Aims: The study sought to determine if okra (abelmoschus esculentus) seed oil was effective in inhibiting the growth of Staphylococcus aureus and Escherichia coli through disc diffusion test.
Study design: The experimental method of research was used in the study.
Place and Duration of Study: University of Perpetual Help-Dr. Jose G. Tamayo Medical University. Between December 2016 and March 2018
Methodology: The seeds were weighed and subjected for the Soxhlet method of extraction using ethanol. The okra seed oil was subjected for phytochemical screening. The assay plates were subjected to disc diffusion test for determination of antimicrobial activity.
Results: The okra seed oil has produced no zone of inhibition (6mm) against the test organisms namely S. aureus and E. coli from the first trial up to the fifth trial, thus the test organisms were resistant and the oil has no effectiveness against the two microorganisms. In contrary to the zones of inhibition produced by the postive control for each organism namely Cefoxitin and Cefixime which produced larger zone of inhibition and has better antibacterial effect for the two microorganisms.
Conclusions: Based on the salient findings, the test organisms namely S. Aureus and E. coli involved in this study were resistant to the okra seed oil. The okra seed os is not comparable to the readily available drug treatment Cefoxitin and Cefixime. The saponins and tannins constituent of the okra were unable to elicit antimicrobial effect alone to the test organism thus synergism with another drug is needed. The polyunsaturated fatty acids component of okra was unable to elicit antibacterial effect due to certain virulence factors and resistant mechanisms of the test microorganisms involved in the study.