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HERDIN Record #: NCR-RITM-12081316243520 Submitted: 14 August 2012 Modified: 07 February 2013

Flow cytometric analysis of dengue virus infected cells in peripheral blood.

Michael O. Baclig,
Lady-Anne C. Suarez,
Corazon C. Buerano,
Ronald R. Matias,
Atsushi Kumatori,
Shingo Inoue,
Kouichi Morita,
Filipinas F. Natividad,
Futoshi Hasebe

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With the development of permeabilization techniques in flow cytometry and the availability of various monoclonal antibodies (MAbs) that specifically bind with cell surface and intracellular antigens, it is now possible to use flow cytometric assay to identify dengue virus (DEN) infected cells in peripheral blood. Blood samples were analyzed using phycoerythrin (PE) labeled anti-CD3, anti-CD14, anti-CD16, and anti-CD19 antibodies and Alexa Fluor® 488 labeled anti-flavivirus monoclonal antibody (MAb) 6B6C-1. The predominant DEN-infected cells were CD19 + in this study. There was dim partial to moderately bright partial expression of CD19 positive cells in the blood samples tested. Virus isolation and serotype-specific RT-PCR revealed the cells were infected with dengue serotype 3 (DEN3). Our results suggest B cells may play an important role in DEN1 and DEN3
replication, and dissemination in vivo.

Publication Type
Journal
Publication Sub Type
Journal Article, Original
Title
St. Luke's Journal of Medicine
Frequency
Bimonthly
Publication Date
January-June 2010
Volume
6
Issue
1
Page(s)
96
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